inno-lipa hbv genotyping  (Fujirebio Inc)

Journal: Frontiers in Immunology

Article Title: Cell-autonomous co-stimulatory function of membrane-bound CD100 promotes activation and differentiation of HBcAg-specific CD8 + T cells

doi: 10.3389/fimmu.2026.1826036

Figure Lengend Snippet: CD100 deficiency impairs proliferation, activation, and effector function of HBcAg-specific CD8 + T cells upon antigen stimulation in vitro . (A) Experimental scheme: Splenocytes from WT C93-TCR-transgenic and CD100KO C93-TCR-transgenic mice were stimulated with Cor93 peptide for 96 hours. (B) The absolute number of CD8 + T cells at each time point are shown. (C–F) Expression levels of Ki67, CD25, Eomes and T-bet in CD8 + T cells were analyzed by flow cytometry. (G) Frequency and mean fluorescence intensity (MFI) of IFN-γ in CD8 + T cells are displayed. Data are depicted as arithmetic means ± SEM. Differences between two groups were analyzed using unpaired Student’s t tests. KO, knockout.

Article Snippet: HBV Cor93 TCR-transgenic (C93-TCRtg) mice were purchased from the Jackson Laboratory.

Techniques: Activation Assay, In Vitro, Transgenic Assay, Expressing, Flow Cytometry, Fluorescence, Knock-Out

Journal: Frontiers in Immunology

Article Title: Cell-autonomous co-stimulatory function of membrane-bound CD100 promotes activation and differentiation of HBcAg-specific CD8 + T cells

doi: 10.3389/fimmu.2026.1826036

Figure Lengend Snippet: mCD100 deficiency results in decreased proliferation and effector function of HBcAg-specific CD8 + T cells upon antigen stimulation in vitro . (A) Experimental setup: CD8 + T cells were isolated from WT C93-TCR-transgenic and CD100KO C93-TCR-transgenic mice, mixed at a 1:1 ratio, and cocultured with WT non-CD8 splenocytes in the presence of Cor93 peptide for 96 hours. (B) Frequency of CD8 + T cells. (C–E) Ki67, Eomes and T-bet expression on CD8 + T cells were detected by flow cytometry. (F–G) Frequencies and mean fluorescence intensity (MFI) of IFN-γ and Granzyme B in CD8 + T cells are shown. Data are depicted as arithmetic means ± SEM. Differences between two groups were analyzed using unpaired Student’s t tests. MACS, Magnetically Activated Cell Sorting; KO, knockout.

Article Snippet: HBV Cor93 TCR-transgenic (C93-TCRtg) mice were purchased from the Jackson Laboratory.

Techniques: In Vitro, Isolation, Transgenic Assay, Expressing, Flow Cytometry, Fluorescence, FACS, Knock-Out

Journal: Frontiers in Immunology

Article Title: Cell-autonomous co-stimulatory function of membrane-bound CD100 promotes activation and differentiation of HBcAg-specific CD8 + T cells

doi: 10.3389/fimmu.2026.1826036

Figure Lengend Snippet: Characterization of transcriptomic alterations in CD100KO HBcAg-specific CD8 + T cells. (A) Experimental setup: Splenocytes from WT and CD100KO C93-TCR-transgenic mice were stimulated with Cor93 peptide for 48 hours, followed by CD8 + T cell isolation for RNA sequencing. (B) The number of DEGs between the two groups. (C) Top 20 KEGG enriched terms for significant regulated genes between two groups. (D) Heatmap depicting the expression levels of significant DEGs involved in the “cytokine-cytokine receptor interaction” KEGG pathway. (E) GO biological process terms for the top 20 hub genes (with highest connectivity) from the PPI network of DEGs in the “cytokine-cytokine receptor interaction” pathway. (F) GSEA reveals significant downregulation of gene sets associated with the PI3K-Akt, mTOR, NF-κB, and JAK-STAT signaling pathways in CD100KO CD8 + T cells. DEGs, differentially expressed genes; GSEA, gene set enrichment analysis; PPI, protein-protein interaction; FDR, false discovery rate; NES, normalized enrichment score.

Article Snippet: HBV Cor93 TCR-transgenic (C93-TCRtg) mice were purchased from the Jackson Laboratory.

Techniques: Transgenic Assay, Cell Isolation, RNA Sequencing, Expressing, Protein-Protein interactions